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Climate Change: Shifting Wildlife Disease Dynamics

By Linzy Jauch

Emerging infectious disease threatens biodiversity as it causes significant ecologic shifts in response to a changing climate. Climate change provides opportunities for infectious disease to emerge through elevated mean temperatures, extreme temperature variation, and altered global patterns of precipitation 4,8. Environmental change has the potential to alter physiological and ecological traits of disease such as host-pathogen interactions, pathogen resistance and range distribution. Recent discoveries in wildlife diseases such as Batrachochytrium dendrobatidis (Bd) in amphibians 2 or blue tongue virus (BTV)4 in ruminants is allowing us to understand to the extent in which climate change is altering disease.

Bd infection of the skin causing death through cardiac arrest. Photo: SOLVIN ZANKL/ VISUALS UNLIMITED/ CORBISFI

Today, one-third (32.5%) of the Earth’s amphibians are threatened with extinction6. The pathogenic fungus Batrachochytrium dendrobatidis has been tied to the extinction of 50-80 species worldwide 2 and continues to threaten global amphibian biodiversity. The fungus attacks the moist skin of amphibians and causes degeneration15. Amphibians use their skin in respiration and in balancing essential ions13 within the body. The fungus creates an imbalance in essential ions within the body that result in death through cardiac arrest13. Though rates of infection have been increasing, Bd is not new to species rich environments7, amphibian immune systems were just previously able to fend off infections.

Climate change has altered the disease dynamics of Bd, specifically host-pathogen interactions and pathogen resistance4. Amphibians are ectothermic, relying on external sources to regulate their body temperature and regulate bodily processes such as metabolism and immune responses. Increased climate variability results in temperature fluctuations that subject amphibians to thermal stress. Documented outbreaks of Bd coincide with dramatic changes in temperatures over a short period of time, characteristic of climate change10.   Ectothermic species are more susceptible to thermal stress at temperatures in which they are unaccustomed2 due to an inability to quickly acclimate and match their body temperature with the envrionment4. When temperature changes rapidly, there is an acclimation lag in which the amphibian is adjusting bodily functions to coincide with the temperature shift. However, microbes and pathogens have a broader range of temperature tolerances2 and acclimate quickly8. This thermal mismatch creates a window of opportunity for infection that was less frequent before human-caused climate change caused increased regional temperature variation. Monthly and diurnal fluctuations in temperature decreases amphibian resistance to Bd by suppressing immune defenses that are relient on the amphibian’s ectothermic physiology. Together, through a slow acclimating immune response and a suppressed immune system, Bd is becoming an epidemic.

While Bd’s emergence is in response to changes in host-pathogen interactions, other infectious diseases such as blue-tongue virus in ruminants has undergone a different type of shift in response to climate change. The disease is transferred though the bites of cold-sensitive midges and has been responsible for mass mortalities of sheep and cattle in the Mediterranean region14. Higher average temperatures have led to the northward expansion of midges, and thus an expansion of the BTV range4. The expansion provides new opportunities for the infection of new hosts and threatens domestic and wild ruminant biodiversity.

Regions in blue demonstrate areas now suitable for BTV based on current climate data and increased average temperatures allowing range expansion. Original ranges in North Africa and in the Middle East (4). Chart by Samy and Peterson 2016.

Disease dynamics are altering in response to human-caused climate change7 and are contributing to more extinctions than we originally suspected. The link between human-mediated climate change and infectious disease is complicated due to many intertwining factors5 and must be teased apart to understand what can be done to prevent future emerging infectious diseases. Infectious disease is significantly impacting complex ecologic processes. Biodiversity loss, abandoned niches, and new host infections have already resulted as a response to climate change and infectious disease emergence. Future research focused on understanding the extent to which biodiversity loss has been magnified through infectious disease emergence is necessary for developing mitigation methods that may save small and endangered wildlife populations from extinction.

References

[1] Altizer S, Ostfeld RS, Johnson PT, Kutz S, Harvell CD. 2013. Climate Change and Infectious Diseases: From Evidence to a Predictive Framework. Science 341: 514 – 519.

[2] Cohen J. 2016. Climate Change Drives Outbreaks of Emerging Infectious Disease and Phenological Shifts. Unpublished PhD thesis, University of South Florida, Tampa, USA.

[3] Daszak P, Cunningham AA, Hyatt AD. 2001. Anthropogenic environmental change and the emergence of infectious diseases in wildlife. Acta Tropica 78: 103-116.

[4] Gallana M, Ryser-Degiorgis MP, Wahli T, Segner H. 2013. Climate change and infectious diseases of wildlife: Altered interactions between pathogens, vectors and hosts. Current Zoology 59(3): 427-437.

[5] Lafferty KD. 2009. The ecology of climate change and infectious diseases. Ecology 90(4): 888-900.

[6] Lips KR, Brem F, Brenes R, Reeve JD, Alford RA, Voyles J, Carey C, Livo L, Pessier AP, Collins JP. 2006. Emerging infectious disease and the loss of biodiversity in a Neotropical amphibian community. PNAS 103(9): 3165-3170.

[7] Pounds JA, Bustamante MR, Coloma LA, Consuegra JA, Fogden MPL, Foster PN, Marca EL, Masters KL, Merino-Viteri A, Puschendorf R, Ron SR, Sánchez-Azofeifa, Still CJ, Young BE. 2006. Widespread amphibian extinctions from epidemic disease driven by global warming. Nature 439: 161-167.

[8] Raffel TR, Halstead NT, McMahon TA, Davis AK, Rohr JR. 2015. Temperature variability and moisture synergistically interact to exacerbate an epizootic disease. Proceedings of the Royal Society B 282(1801).

[9] Raffel TR, Romansic JM, Halstead NT, McMahon TA, Venesky MD, Rohr JR. 2013. Disease and thermal acclimation in a more variable and unpredictable climate. Nature Climate Change 3: 146-151.

[10] Rohr JR and Raffel TR. 2010. Linking global climate and temperature variability to widespread amphibian declines putatively caused by disease. PNAS  107(18): 8269-8274.

[11] Rohr JR, Raffel TR, Romansic JM, McCallum H, Hudson PJ. 2008. Evaluating the links between climate, disease spread, and amphibian declines. PNAS 105(45):17436-17441.

[12] Rohr JR, Schotthoefer AM, Raffel TR, Carrick HJ, Halstead N, Hoverman JT, Johnson CM, Johnson LB, Lieske C, Piwoni MD, Schoff PK, Beasley VR. 2008. Agrochemicals increase trematode infections in a declining amphibian species. Nature 445(30): 1235 -1239.

[13] Rollins-Smith LA. 2017. Amphibian immunity – stress, disease, and climate change. Developmental and Comparative Immunology 66:111-119.

[14] Samy AM and Peterson AT. 2016. Climate Change Influences on the Global Potential Distribution of Bluetongue Virus. PLoS ONE 11(3).

[15] Vredenburg VT, Knapp RA, Tunstall TS, Briggs CJ. 2010. Dynamics of an emerging disease drive large-scale amphibian population extinctions. PNAS 10(21): 9689-9694.

[16] Yang Xie G, Olson DH, Blaustein AR. 2016. Projecting the Global Distribution of the Emerging Amphibian Fungal Pathogen, Batrachochytrium dendrobatidis, Based on IPCC Climate Futures. PLoS ONE 11(8).

 

 

 

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A New Way to Combat Introduced Diseases: The Use of Synthetic Biology in Conservation – By Feyza Zaman

The current ecological atmosphere is arguably one of instability, over-exploitation, and a rapid and poorly understood change towards global homogeneity. The numerous anthropogenic pressures placed on the natural environment, potentially beginning with early humans millennia ago, have been ever increasing in volume and effect, with extinction rates now thought to be approximately 100-1000 times greater than what is considered normal background levels (Smith et al. 2009). These ongoing impacts are now thought to be leading towards a sixth mass extinction of biota on earth, comparable in magnitude to the Cretaceous-Palaeogene extinction which wiped out the dinosaurs (Raup and Sepkoski 1982; Barnosky et al. 2011). Currently, the negative impacts which human activities have upon biodiversity can be broadly placed into four categories; habitat destruction, over-exploitation, the introduction of non-native species, and the subsequent spread of diseases caused by pathogens carried by these species (Wilcove et al. 1998; Smith et al. 2009). Although the first three of these impacts have been found by many studies to have the greatest influence upon the abundance and diversity of native communities (e.g. Venter et al. 2006), it is also acknowledged that the role of introduced diseases is not yet fully understood. However, it is now coming to light that such diseases may pose a much greater risk than previously thought, and will require the development of new methods of treatment and conservation to deal with these threats (Smith et al. 2006; Smith et al. 2008; Fisher et al. 2012).

There has been an apparent increase in the emergence of infectious diseases in wildlife in recent times, and there is substantial evidence that these outbreaks have been greatly affecting local species populations by causing severe and potentially permanent declines in abundance (Harvell et al. 2002; Smith et al. 2006; Smith et al. 2008; Fisher et al. 2012). Factors thought to be contributing to this increase include changes in environmental dynamics, such as increased susceptibility from stress-causing temperature fluctuations; ecological influences, such as reduced genetic diversity from other factors like habitat loss; and socio-economic factors, including globilisation and increasing mobility resulting in more introduced species (Smith et al. 2006; Jones et al. 2008; Smith et al. 2012). There are many pathogens which are known to result in widespread reductions in wildlife diversity, however much attention has recently been placed on several fungal agents of disease which are known to have resulted in the ecological collapse of formerly highly abundant species, and a prominent example of this is blight in the American chestnut (Fisher et al. 2012; Powell 2014). In this review, we will be focusing on the chestnut blight as a case study, outlining the potential processes necessary for advising further action in other disease epidemics which have appeared worldwide.

With these relatively new challenges to biodiversity conservation have come numerous approaches attempting to resolve them, ranging from traditional methods to the application of technologies novel to conservation biology. One such novel partnership of disciplines is that of synthetic biology and conservation biology. Synthetic biology involves the manipulation, design, and construction of chemically synthesised DNA or other biological constructs with the aim to meet “human needs by the creation of organisms with novel or enhanced characteristics” (Presidential Commission for the Study of Bioethical Issues 2010; Redford et al. 2013). In the case of disease control, synthetic biology may allow us to more intimately understand the causal agents, potentially leading to methods of reducing their pathogenicity, or to introduce protective alleles into susceptible species. So, whilst traditional methods are still required to support and supplement conservation, they have clearly not achieved their goal of halting biodiversity loss (Butchart et al. 2010). Thus new technologies such as transgenesis, synthetic biology techniques, and DNA manipulation may provide the next step (Redford et al.2013; Powell 2014). These ideas have only in recent years been brought together with conservation biology, and may have the potential to greatly widen the scope of biodiversity protection.

Natural pre-blight range of the American Chestnut across the eastern USA, exceeding 800,000km2 (adapted from Jacobs 2007).

Natural pre-blight range of the American Chestnut across the eastern USA, exceeding 800,000km2 (adapted from Jacobs 2007).

A prominent example of the application of these novel techniques is in the American chestnut tree (Castanea dentata), whose widespread populations were decimated by an introduced fungal blight (Wheeler and Sederoff 2009; Zhang et al. 2013). Before the early 20th century, a quarter of the hardwood forests of the eastern United States constituted of American chestnut, numbering in the billions (Merkle et al. 2007; Powell 2014). This tree was a keystone species in its ecosystem, contributing immensely to forest structure and complexity, and providing habitat and a stable food source for squirrels, bears, deer, and many other animals (Powell 2014). Deliberate introductions in the early 1900s of the Japanese (Castanea crenata) and Chinese (C. mollissima) chestnut trees brought with them spores of the pathogenic fungus Cryphonectria parasitica, the causal agent of chestnut blight (Barakat et al. 2009; Powell 2014). These trees, having evolved with the fungus, had a natural resistance to it; however the American variant did not, never having been exposed to it. Establishing itself below the bark, the blight spreads filamentous hyphae into the tree and produces oxalic acid, among other toxic substances, which lowers the pH of infected tissue to lethal levels. As the toxins spread, a canker forms around the trunk or limb of the tree, and eventually cuts off extremities from water and nutrients, resulting in death (Anagnostakis 1987). This disease ran rampant through the north-eastern populations of American chestnut, killing more than 3 billion individuals within 50 years (Powell 2014). The American chestnut is now functionally extinct within its natural range, with very few adult trees left in various areas around America, mostly outside this natural distribution. It is important to note that, along with the important ecological roles that this species fulfilled, the American chestnut was also very important economically, providing food, fuel, and fast growing and rot resistant timber (Homles et al. 2009).

Different stages of blight disease in American Chestnut (Taken from Anagnostakis 1982 and Powell 2014).

Different stages of blight disease in American Chestnut (Picture credits: Anagnostakis 1982 and Powell 2014).

The imperative nature of the situation was recognised very early on, and over the decades much research has been directed towards mitigating the effects of the blight. There are currently three strategies underway which appear to be having some measure of success: one utilising ancient horticulture cross- and back-breeding techniques; one targeting the fungus directly through an introduced virus; and one utilising genetic engineering to insert specific strands of DNA into the tree’s genome (Grüenwald 2012; Zhang et al. 2013; Powell 2014). The first method is focussing on hybridising the American chestnut with Asian variants, and further back-breeding with the American species in order to create individuals with blight-resistance, whilst retaining as many of the American species’ traits as possible (Diskin et al. 2006; Hebard 2006; Jacobs 2007). However, this method can be very inexact, and requires many generations to achieve the desired results. Projects using this method have spanned over several decades, and there has been some success with several re-plantings of hybrid trees across America (Diskin et al. 2006; Hebard 2006; Jacobs 2007). However, the genes for resistance from the Asian trees is incompletely dominant, and intensive selection through individual maturation, inoculation with the disease to check for resistance, and subsequent crossing of resistant individuals is required (Powell 2014).

The second method involves targeting the blight fungus directly with hypoviruses (a virus that affects only fungi) from the family Hypoviridae. These hypoviruses have the very specific effect of reduced virulence and sporulation of the blight fungus, but has been found to be almost too efficient, commonly killing the fungus on a single tree before the virus is able to spread to other infected trees (Kazmierczak et al. 1996). Although effective if an infection is caught early, this method requires inoculation of individual trees and can be labour and time intensive.

Using modern technology, the third method is attempting to confer disease resistance directly and in a much more precise manner, targeting specific symptoms of the fungus on the trees. Using the natural DNA transmission capabilities of the Agrobacterium tumefaciens bacteria, scientists at the State University of New York (SUNY) were able to transfer genes from a species of wheat resistant to an oxalic acid producing fungus similar to the chestnut blight, Sclerotinia sclerotorium (Welch et al. 2007; Zhang et al. 2013; Powell 2014). The gene codes for the enzyme oxalate oxidase – which chemically breaks down oxalic acid – appears to be working exceptionally well in the transgenic American chestnut trees. Several generations of the modified trees have been created, with varying levels of oxalate oxidase production within their cells, and several strains appear to even be more resistant to the blight than the Asian species (Zhang et al. 2013; Powell 2014). The team at SUNY has stated that as their research continues they intend to introduce further lines of defence against the fungus to safeguard against potential future adaptation by the pathogen, and include enzymes from various other plants to confer less specific anti-fungal properties (Zhang et al. 2013; Powell 2014).

The American Chestnut's distinctive features, from a mature tree. Picture credit: American Chestnut Foundation

The American Chestnut’s distinctive features, from a mature tree. Picture credit: American Chestnut Foundation

The next hurdle to such technological advancement in conservation biology is not scientific, but social and political acceptance. Although genetically modified crops and products have mostly become commonplace amongst our modern societies – although not completely, with notable anti-genetically modified organism movements – the prospect of genetically altering wildlife with the intention of releasing them may require further consideration (Dana et al. 2012; Redford et al. 2013;Powell 2014). Having achieved their initial goals of creating a disease resistant species, acquiring official permission from the Food and Drug Administration and US Department of Agriculture is seen as the next major obstacle by the team at SUNY. With the American chestnut tree having both an important role in ecosystem processes, and high economic value, it is believed that this approval will be achieved within the next 5 years (Powell 2014).

If such a precedent is set, it may pave the way for much wider applications of this technology in the service of conservation biology. There are similar situations to the American chestnut where entire species or even higher taxonomic orders have been subjected to enormous reductions in abundance due to introduced fungal diseases, including the global epidemic of Chytrid disease in amphibians, and the more recent outbreaks of white-nose syndrome among American bats (Weldon et al. 2004; Frick et al. 2010). Both of these diseases have reached pandemic proportions, and tireless research still has yet to provide a solution. Although fundamentally different from the concepts discussed above, which primarily apply to plant ecology, the overall conclusions can mostly be applied to these amphibian and mammalian taxa. The causative agents of the fungal diseases, as well as their symptoms, have been identified – however effective methods of combatting these remain elusive. When facing global dissemination, such as the Chytrid disease, or up to 95% mortality rates as in the White-nose syndrome, urgent action needs to be taken in order to manage and minimise biodiversity loss (Weldon et al. 2004; Frick et al. 2010; Woodhams et al. 2012). If genes of resistance to such diseases can be identified in alternate organisms, and further research is undertaken in biosynthetic and transgenic techniques, it is just a matter of time before the processes detailed in this discussion become applicable to a wider range of pathogens for conservation related purposes.

References

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